8: theMicroplate Reader & Luminometer Selection Guide. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. I am using the filter pair of 480/535 and trying to measure fluorescence. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. Reader Documentation Version No. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. BioTek Synergy 2. flexibility of monochromator-based systems. Double-click the unknown device with the yellow warning icon. This reader's dual monochromators for. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. We therefore gathered salient. Add to Cart. The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. U. Compact and tunable absorbance microplate readers DATA SHEET. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. Figure 6: Transcreener ADP2 FI assay principle. Appendix A. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Absorbance; Fluorescence (FI, TR, FP) Luminescence. Biotek Synergy 2 Plate Reader Multi-Mode; Related products. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. It can measure fluorecence from the top or bottom of a plate with a range of 250 - 850 nm. ~ Bio-Tek ELx808 Microplate Reader $2,795. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Luminex Magpix. $ 2,499. Appendix. On microplate readers,. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. CO 2 & O 2 control. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. 0 out of 5 $ 14,499 $ 13,995-13%. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The method is based on. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). B. The SpectraMax microplate readers support a wide variety of assay categories including . Products : SpectraMax M2/M2e reader, SpectraMax. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. type: Clear: Add to Cart. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. both cuvette-based and microplate. com 10. View online or download Molecular devices SpectraMax M5 User Manual. Page 28 Chapter 5: Read Modes and Read Types 1. Reading Capabilities: cuvette or microplate. Download Application Note Plate reader settings for NanoOrange assay. 0 out of 5 $ 2,999-20%. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. spr SpectraMax iD5 SpectraTest ABS2 v1. BMG Labtech - THERMOstar. It can measure absorbance from 230 -1000 nm. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Tecan’s. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. Measure more accurately. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The purple holder is used for reading non-transparent plates from the top (e. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. plate over and over again. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Perkin Elmer 1420-2560 Victor X Series Plate Reader. 306 plate air cal fail,low light. Optical System: High energy xenon flash lamp. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 2. g. Fluorescence intensity values increased over time, but similar EC 50 values of 263 nM and 295 nM were obtained for assay incubation times ranging from one. spr SpectraMax Plus 384 SpectraTest ABS2 v1. Fluorescence read mode and Endpoint read type are already selected in the pre-configured protocol. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Third this recommendation. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. spr SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. 5). Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 00. SpectraMax M5), place the adapter in the plate carriage. Update Driver. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. 9000-0756. Category 2 and 3 microplate readers use gradient heating as well. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Downloads. Danaher Inc spectramax m2 fluorescence microplate reader Spectramax M2 Fluorescence Microplate Reader, supplied by Danaher Inc, used in various techniques. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. Learn how you can utilize microplate readers to drive landmark discoveries. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Shop all used microplate readers and parts from a variety of manufacturers. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). The chamber. (See Figure 7. 0 out of 5 $ 9,995 $ 9,495. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 6 % of reading 4. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax Quant dsDNA Assay Kits. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 67. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Flexibility to read 6- to 384-well plates. Plate reader. 7°C and the bottom to 36. LuminescencePhotometricPerformance. At different time points, the plate was removed from the incubator and loaded to the plate reader for fluorescence reading (excitation 450 nm,. 5%in96-and384-wellmicroplates. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. Biotek FLx800 Fluorescence Microplate Reader. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. Microplate Reader, Multimode. The chamber. 8 Customer Reviews. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. I understand the OD measurement in a plate reader varies with the volume of the sample measured. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. Figure 2. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. 0077 OD 0. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. A. 1. g. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Absorbance (monochromator). Plate background OD values of disposable UV microplates vary slightly between lots. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. E Models. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). After the plate is read, data will appear in the plate. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Offer Ends In: 40 Days 05: 02: 03. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. Qualify the fluorescence performance of the SpectraMax . Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Enter wavelength settings as shown below: 4. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 67. Plate Formats: 6, 12, 24, 48, 96, 384 wells: Light Source: Xenon Flash Lamp (1 joule/flash)The SpectraMax® iD3 Multi Mode Microplate . 100 readings per well gives optimal Z’ values for this assay. S. A solution for low volume applications. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. The SpectraMax iD3 reader detects fluorescence using monochromators only. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 87. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. e. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. as a fluorescence blank. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. Add to Cart. made for this assay plate. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Three kits covering a wide range of dsDNA concentrations. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. 58% for the detection module and 2. Transcreener ® ADP. This can increase throughput and efficiency, thus saving time and space in the lab. $22,500. SpectraMax ABS Plus SpectraTest ABS2 v1. Figure 1. Customer Satisfaction is. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. 0 out of 5 $ 2,999. Because. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. FDSS µCell is a whole plate imager that measures fluorescence or luminescence from all wells of microplate continuously and simultaneously with built-in 96/384 channel liquid handling robotics. 3 foot) See. FI assay. It is particularly powerful when monitoring fast kinetic assays in a high. 0 out of 5. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. The IC50s by cell rounding and LysoTracker assays were 0. A solution for low volume applications. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. The modular platform allows upgrading to expand functionality. The Spectr. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. Choose from a three- (M3), four- (M4), orThere were 12 replicate wells per dilution in both 96-well and 384- well plates. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. The following general steps are taken with our absorbance and fluorescence readers. Fully tested with validations and warranty. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and. . Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The systems have. NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 1. 1. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. SpectraMax Paradigm Multi-Mode Safety Guide The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Molecular Devices SpectraMax M2 Multimode Plate Reader. The dual monochromator optics allow the. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. 47 and 0. 53 - 60 kg, (117-132 lb), depending on configuration. Appendix. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. $16,000. These robust readers have been placed in labs from Antarctica to the. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Filters: Excitation and emission slides for 4 filters each. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. 10. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. D 59 A. Fluorescence Microplate Reader Comparison Chart. Other technologies such as. Capture flash assays with ease Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system withThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Tecan Infinite F200 Fluorescence Microplate Reader. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. 5,112,134 5,766,875. Microplate Spectrophotometers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. SpectraMax M2, M2e;. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. 020 OD 2. duAl monochromAtors With SpectraMax M2/M2e. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 10. When connecting for the first time to either the SpectraMax L reader or StakMax system. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. 627. Maximum signal with lowest background. 25 to 3. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. Molecular Devices SpectraMax M2 Microplate Reader. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. 0 out of 5 $ 3,895. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. SpectraMax M2 and use thereof is covered by issued (U. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. fluorescence intensity (Figure 6). Description 1. 0 × 103 to 2. The SpectraTest fluorescence test plate (FL-1) can be used by Gemini XPS & M2 readers. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. It may be named "Pothos". This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Plate reader set to 50 reads. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). NanoQuant plate. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. ID: MOLECULAR DEVICES SpectraMax M2 is a multi-mode microplate spectrophotometer providing unbeatable accuracy, dependability and versatility for any lab experiment, with a range of detection modes, advanced accessories, and intuitive software. Wide range of applications. E Models. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. okyo M LLC O T rinted in in rinted P. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. 3: The cuvette chamber. Basic "FL Plate Reader start up protocol" (print and bring along for the. Temperature variation ± 0. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. for the plate. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. Endpoint, kinetic, spectrum. 7. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. Type. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 7°C (gradient heating design). Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. 2018. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. M2. 3: The cuvette chamber. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 1. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Spectramax Multi-Mode Microplate Reader Performance Specifications. Bottom Monochromator: Fluorescein 2. 00 USD. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. We would like to show you a description here but the site won’t allow us. Plate Readers. Transcreener ® ADP. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. Microplate Reader, For Lab. Read the microplate Step 1: Place the microplate in the reader. . USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Temperature control range from Ambient +4º to 50ºC. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 00 USD. 304 signal level saturation. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Search: Submit search Search filters Search This site Search Cornell. $ 9,499. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. the reader’s stated range. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. standard spectral absorbance, fluorescence, and luminescence detection. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 00 CAD. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Wavelength optimization.